[Amethyst_ice]

I can perhaps answer that too as in the rat community, many people (attempt) home CO2 chambers due to either breeding, numbers or own ethics.

However, while it is very appealing it has a HUGE margin of error and when you get it wrong, it is one of those horrible situations where you have to keep going and then watch your pet suffer.

Trying to get the concentrations right to not burn the lungs or cause suffocation is hinged on many factors such as tank size, concentration and release time. It really is very tricky to get right.

Only once has a breeder allowed me to take my rat to her to use her tank. She did it and when I got my rat back his eyes were like mercury. It did not sit well with me.

As such, I think it is wise to avoid any home attempt at euthanasia