[souffle]

No you can't put them back together ever. They are territorial and the female has decided this is her place. It is not good for them to be together and breeding back to back litters either.
They are not small people and they do not need family playtime. In the wild the females would drive away the young and start her next litter. You need to separate them in to male and female groups and keep them separate - no play dates or any other interaction. Find homes for the pups as singles or same sex pairs or else they will mate with each other and then you have inbred animals which could have medical problems.
Please for your hamsters sakes keep the sexes separate. You will end up with hundreds of hamsters in no time at all if you don't.